Reporter

Part:BBa_J100262:Design

Designed by: Zachary Shaver   Group: Campbell M Lab   (2016-06-08)


rClone Red with Riboswitch E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 655
    Illegal AgeI site found at 767
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The sequence for riboswitch E was taken from the Gallivan et al. paper "A family of synthetic riboswitches adopts a kinetic trapping mechanism" (2014). The rClone Red plasmid used in GGA is part BBa_J119384.

References